Sort of, yes. It's more about the words used than the accent. That is to say that it can sometimes be hard for me to place you as Canadian or a Wisconsinite/Minnesotan unless you're appending sentences with the stereotypical "eh?" or pronouncing words like 'again' as 'ay-gain' or 'about' as 'ah-boot' -- any of which pretty much out you as Canadian. Conversely, things like "dontchaknow?" or "bubbler" or the infamous "ope! sorry!" (pronounced anywhere between 'oupe' and 'ouwhup' depending on the speaker) instantly out you as a Midwestern American.
Normally, though, the accents are different enough to tell a Canadian apart from a Midwesterner. Although it's my understanding that you have to have lived here for a while to be able to tell the difference. I'm not originally from here, but I've been in the Midwest off and on since the early eighties, so I probably take it for granted by now.
Edit: For clarity, lemmie just say that you're only going to be potentially confusing Canadians with Midwestern Americans. This isn't going to happen with any other region of the country. New Englanders are, at best, "Canadian-adjacent" sounding. People from the West Coast (SoCal in particular) are never going to be mistaken for Canadians. Neither are Southerners. (And there are some accents in Texas, Tennessee, and Louisiana that aren't even pronounceable with human vocal cords. Make of that what you will.)